KLHL34 Polyclonal antibody

KLHL34 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21981-1-AP

Synonyms

kelch like 34 (Drosophila), Kelch like protein 34, KLHL34

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Validation Data Gallery

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  WB analysis using 21981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21981-1-AP (KLHL34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 21981-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21981-1-AP (KLHL34 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 21981-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21981-1-AP (KLHL34 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21981-1-AP targets KLHL34 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL34 fusion protein Ag16001
• Full Name: kelch-like 34 (Drosophila)
• Calculated Molecular Weight: 644 aa, 71 kDa
• Observed Molecular Weight: 71 kDa
• GenBank Accession Number: BC109031
• Gene Symbol: KLHL34
• Gene ID (NCBI): 257240
• RRID: AB_2878959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL34 is a member of the Kelch-like (KLHL) gene family, which encodes a group of proteins that generally possess a BTB/POZ domain, a BACK domain and 5-6 Kelch motifs. KLHL genes are responsible for several Mendelian diseases and have been associated with cancer. The molecular mass of KLHL34 is 71 kDa.