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WB analysis of HeLa using 21191-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21191-1-AP (KLHL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21191-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21191-1-AP (KLHL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 21191-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21191-1-AP (KLHL4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 21191-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 21191-1-AP (KLHL4 antibody) at dilution of 1:50 (under 40x lens).

KLHL4 Polyclonal antibody

Name: KLHL4 Polyclonal antibody
Brand: Proteintech
SKU: 21191-1-AP

KLHL4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21191-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Synonyms

DKELCHL, kelch like 4 (Drosophila), Kelch like protein 4, KHL4, KIAA1687, KLHL4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

21191-1-AP targets KLHL4 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15529 Product name: Recombinant human KLHL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 15-164 aa of BC142652 Sequence: LRLRWRWFSHPFQGSTNTGSCLQQEGYEHRGTPVQGRLKSHSRDRNGLKKSNSPVHHNILAPVPGPAPAHQRAVQNLQQHNLIVHFQANEDTPKSVPEKNLFKEACEKRAQDLEMMADDNIEDSTARLDTQHSEDMNATRSEEQFHVINH Predict reactive species
Full Name	kelch-like 4 (Drosophila)
Calculated Molecular Weight	720 aa, 81 kDa
Observed Molecular Weight	75-80 kDa
GenBank Accession Number	BC142652
Gene Symbol	KLHL4
Gene ID (NCBI)	56062
RRID	AB_10755294
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9C0H6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for KLHL4 antibody 21191-1-AP	Download protocol
WB protocol for KLHL4 antibody 21191-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,CoIP	Med Oncol KLHL4 upregulates EGFR signaling to promote the malignant progression of oral squamous cell carcinoma. Authors - Yingxin Zhang KD Validated View Article

WB analysis of HeLa using 21191-1-AP