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WB analysis using 32697-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32697-1-AP (KLHL7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis using 32697-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32697-1-AP (KLHL7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

KLHL7 Polyclonal antibody

KLHL7 Polyclonal Antibody for WB, ELISA

Cat No. 32697-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Kelch-like protein 7

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

mouse brain tissue, mouse cerebellum tissue, mouse testis tissue, rat brain tissue, rat cerebellum tissue, HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32697-1-AP targets KLHL7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag38050 Product name: Recombinant human KLHL7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 291-586 aa of BC039585 Sequence: HDYRIALFGGSQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYNVVKDSWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQRCSHGMVEANGLIYVCGGSLGNNVSGRVLNSCEVYDPATETWTELCPMIEARKNHGLVFVKDKIFAVGGQNGLGGLDNVEYYDIKLNEWKMVSPMPWKGVTVKCAAVGSIVYVLAGFQGVGRLGHILEYNTETDKWVANSKVRAFPVTSCLICVVDTCGANEETLET Predict reactive species
Full Name	kelch-like 7 (Drosophila)
Calculated Molecular Weight	586 aa, 66 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC039585
Gene Symbol	KLHL7
Gene ID (NCBI)	55975
RRID	AB_3742714
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q8IXQ5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL7 (Kelch-like protein 7) is a member of the Kelch-like protein family and plays significant roles in various biological processes, including cell cycle regulation, protein degradation, and cytoskeleton organization. KLHL7 is highly expressed in various cancers and is associated with malignant progression and poor prognosis. In breast cancer, high expression of KLHL7 is linked to higher histological grades, estrogen receptor (ER)-negative or human epidermal growth factor receptor 2 (HER-2)-positive tumors, and triple-negative breast cancer (TNBC), and patients with high KLHL7 expression have shorter survival times. In gastric cancer, silencing KLHL7 gene expression can inhibit the proliferation, migration, and invasion of gastric cancer cells and has shown tumor-suppressive effects in in vivo models. Moreover, KLHL7 is highly expressed in colorectal cancer and ovarian cancer, among others, and is significantly associated with low patient survival rates.