Kallikrein 11 Polyclonal antibody

Kallikrein 11 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12698-1-AP

Synonyms

Hippostasin, hK11, Kallikrein 11, KLK11, PRSS20, Serine protease 20, TLSP, Trypsin like protease

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Validation Data Gallery

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  WB analysis of MCF-7 using 12698-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12698-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12698-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of PC-3 using 12698-1-AP

  Immunofluorescent analysis of PC-3 cells using 12698-1-AP (Kallikrein 11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells, PC-3 cells, Y79 cells
• Positive IHC detected in: human prostate cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12698-1-AP targets Kallikrein 11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kallikrein 11 fusion protein Ag3427
• Full Name: kallikrein-related peptidase 11
• Calculated Molecular Weight: 250 aa, 27 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC022068
• Gene Symbol: KLK11
• Gene ID (NCBI): 11012
• RRID: AB_2877876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.