KLK3/PSA Monoclonal antibody

KLK3/PSA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 60338-1-Ig


APS, Gamma seminoprotein, hK3, Kallikrein 3, kallikrein related peptidase 3, KLK2A1, KLK3, KLK3,PSA, P 30 antigen, Prostate specific antigen, PSA, Semenogelase, Seminin

Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 6 publications below

Product Information

60338-1-Ig targets KLK3/PSA in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLK3/PSA fusion protein Ag1060
Full Name kallikrein-related peptidase 3
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC005307
Gene Symbol KLK3
Gene ID (NCBI) 354
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. kallikrein-related peptidase 3, which is also called PSA (prostate-specific antigen) in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. KLK3 has 5 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for KLK3/PSA antibody 60338-1-IgDownload protocol
IHC protocol for KLK3/PSA antibody 60338-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Dis

N-glycosylation of GDF15 abolishes its inhibitory effect on EGFR in AR inhibitor-resistant prostate cancer cells

Authors - Rong Wang

J Cell Commun Signal

Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling

Authors - Yalcin Erzurumlu

Cell Signal

1,25(OH)2 D3 induced vitamin D receptor signaling negatively regulates endoplasmic reticulum-associated degradation (ERAD) and androgen receptor signaling in human prostate cancer cells

Authors - Yalcin Erzurumlu

J Nat Med

Potent carotenoid astaxanthin expands the anti-cancer activity of cisplatin in human prostate cancer cells

Authors - Yalcin Erzurumlu

Cell Signal

Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells

Authors - Yalcin Erzurumlu

ACS Appl Mater Interfaces

Two-in-One Polymeric NO-Donor Prodrugs Mediate Precision and Synergistic Prostate Cancer Treatment

Authors - Bingbing Zhao