Kallikrein 7 Polyclonal antibody

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  WB analysis using 23810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23810-1-AP (Kallikrein 7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 23810-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Kallikrein 7 antibody (23810-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Kallikrein 7 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23810-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

KLK7, EC:3.4.21.117, Kallikrein-7, PRSS6, SCCE

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Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23810-1-AP targets Kallikrein 7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20760

  Product name: Recombinant human KLK7 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-253 aa of BC032005

  Sequence: MARSLLLPLQILLLSLALETAGEEAQGDKIIDGAPCARGSHPWQVALLSGNQLHCGGVLVNERWVLTAAHCKMNEYTVHLGSDTLGDRRAQRIKASKSFRHPGYSTQTHVNDLMLVKLNSQARLSSMVKKVRLPSRCEPPGTTCTVSGWGTTTSPDVTFPSDLMCVDVKLISPQDCTKVYKDLLENSMLCAGIPDSKKNACNGDSGGPLVCRGTLQGLVSWGTFPWGQPNDPGVYTQVCKFTKWINDTMKKHR

• Full Name: kallikrein-related peptidase 7
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: BC032005
• Gene Symbol: KLK7
• Gene ID (NCBI): 5650
• RRID: AB_3669417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P49862
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kallikrein-7(KLK7), also known as SCCE, PRSS6, is a member of the kallikrein subfamily of serine proteases involved in various enzymatic processes. KLK7 is abundantly expressed in the skin and is expressed by keratinocytes in the epidermis(PMID: 10974542).