KLRB1/CD161 Monoclonal antibody

KLRB1/CD161 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D8F5

Cat no : 67537-1-Ig

Synonyms

CD161, CLEC5B, hNKR P1A, KLRB1, NKR, NKR P1, NKR P1A, NKRP1A

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Validation Data Gallery

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  WB analysis of human spleen using 67537-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67537-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67537-1-Ig (KLRB1/CD161 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human spleen tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

Sample-dependent, check data in validation data gallery

Product Information

67537-1-Ig targets KLRB1/CD161 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLRB1/CD161 fusion protein Ag27117
• Full Name: killer cell lectin-like receptor subfamily B, member 1
• Calculated molecular weight: 225 aa, 25 kDa
• Observed molecular weight: 32 kDa
• GenBank accession number: BC114516
• Gene symbol: KLRB1
• Gene ID (NCBI): 3820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.