Tested Applications
| Positive WB detected in | human spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
67537-1-Ig targets KLRB1/CD161 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KLRB1/CD161 fusion protein Ag27117 Predict reactive species |
| Full Name | killer cell lectin-like receptor subfamily B, member 1 |
| Calculated Molecular Weight | 225 aa, 25 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC114516 |
| Gene Symbol | CD161 |
| Gene ID (NCBI) | 3820 |
| ENSEMBL Gene ID | ENSG00000111796 |
| RRID | AB_2882756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12918 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLRB1/CD161 antibody 67537-1-Ig | Download protocol |
| IHC protocol for KLRB1/CD161 antibody 67537-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Med Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers | ||
Front Immunol Comprehensive single-cell and bulk transcriptomic analyses to develop an NK cell-derived gene signature for prognostic assessment and precision medicine in breast cancer | ||
J Thorac Dis KLRB1 expression is associated with lung adenocarcinoma prognosis and immune infiltration and regulates lung adenocarcinoma cell proliferation and metastasis through the MAPK/ERK pathway | ||
Front Endocrinol (Lausanne) Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer | ||
BMJ Open Respir Res Single-cell RNA-sequencing uncovers the dynamic changes of tumour immune microenvironment in advanced lung adenocarcinoma | ||
Front Immunol Single-cell sequencing analysis and multiple machine-learning models revealed the cellular crosstalk of dendritic cells and identified FABP5 and KLRB1 as novel biomarkers for psoriasis |
