CoraLite® Plus 647 Anti-Human KLRB1/CD161 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using CL647-98281

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human KLRB1/CD161 Rabbit RecAb (CL647-98281, Clone: 242140H5) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

KLRB1/CD161 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98281

Clone No.242140H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD161, 242140H5, CLEC5B, C-type lectin domain family 5 member B, hNKR P1A

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98281 targets KLRB1/CD161 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KLRB1/CD161 fusion protein Eg1788
• Full Name: killer cell lectin-like receptor subfamily B, member 1
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_002258.3
• Gene Symbol: CD161
• Gene ID (NCBI): 3820
• ENSEMBL Gene ID: ENSG00000111796
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12918
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD161, also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).