CoraLite® Plus 647 Anti-Mouse KLRG1 (2F1)

KLRG1 Monoclonal Antibody for FC

Host / Isotype

Golden Syrian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F1

Cat no : CL647-65084

Synonyms

2F1 Ag, Klrg1, MAFA, MAFA L

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-65084

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse KLRG1 (2F1) (CL647-65084, Clone: 2F1) or 0.25 ug Isotype Control, and 0.25 ug FITC Anti-Mouse NK1.1 (CD161) (PK136) (FITC-65138, Clone: PK136). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65084 targets KLRG1 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Golden Syrian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: killer cell lectin-like receptor subfamily G, member 1
• GenBank Accession Number: BC101953
• Gene Symbol: Klrg1
• Gene ID (NCBI): 50928
• RRID: AB_2920251
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).