KMO Polyclonal antibody

• Featured Product
• KD/KO Validated

KMO Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 10698-1-AP

Synonyms

KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

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Validation Data Gallery

• 

  WB analysis of mouse spleen using 10698-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10698-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 10698-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 10698-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10698-1-AP (KMO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse heart using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10698-1-AP (KMO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10698-1-AP (KMO antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10698-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10698-1-AP (KMO antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10698-1-AP

  Immunofluorescent analysis of HepG2 cells using 10698-1-AP (KMO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMO antibody (10698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KMO antibody (10698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 10698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 10698-1-AP (KMO antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human prostate cancer tissue, human breast cancer tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 9 publications below
• IF: See 10 publications below

Product Information

10698-1-AP targets KMO in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KMO fusion protein Ag1109
• Full Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• Calculated Molecular Weight: 486 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC005297
• Gene Symbol: KMO
• Gene ID (NCBI): 8564
• RRID: AB_2296744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

Publications

Species	Application	Title
mouse	IF	J Hazard Mater; Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease; Authors - Myungkyung Noh
human	WB	Circ Res; Activation of NAD(P)H Oxidase by Tryptophan-Derived 3-Hydroxykynurenine Accelerates Endothelial Apoptosis and Dysfunction In Vivo.; Authors - Qiongxin Wang
human, mouse	IF	J Am Soc Nephrol; Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria.; Authors - Ron Korstanje
human	IHC	J Intern Med; Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans.; Authors - R Baumgartner
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	WB	PLoS Genet; A novel role for kynurenine 3-monooxygenase in mitochondrial dynamics.; Authors - Daniel C Maddison

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (03-23-2022)

works good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Huh6 and Hepg2

KMO Antibody Western Blot validation (1/1000 dilution) in Huh6 and Hepg2 (Cat no:10698-1-AP)

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