Kininogen 1 Monoclonal antibody

Kininogen 1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

10E8D11

Cat no : 66123-1-Ig

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1



Tested Applications

Positive WB detected inhuman heart tissue, human placenta tissue, human plasma tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66123-1-Ig is Kininogen 1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Kininogen 1 fusion protein Ag19233
Full Name kininogen 1
Calculated molecular weight 644 aa, 72 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC060039
Gene symbol KNG1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

Protocols

Product Specific Protocols
WB protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
IHC protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
IF protocol for Kininogen 1 antibody 66123-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols