Filter: AllWBIHCIF

WB analysis of human heart using 66123-1-Ig (same clone as 66123-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 66123-1-Ig (same clone as 66123-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
WB analysis of human plasma using 66123-1-Ig (same clone as 66123-1-PBS)
human blood were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66123-1-Ig (same clone as 66123-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66123-1-Ig (same clone as 66123-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66123-1-Ig (same clone as 66123-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

Kininogen 1 Monoclonal antibody, PBS Only

Kininogen 1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66123-1-PBS

Clone No.10E8D11

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

66123-1-PBS targets Kininogen 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag19233 Product name: Recombinant human KNG1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 20-374 aa of BC060039 Sequence: ESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLG Predict reactive species
Full Name	kininogen 1
Calculated Molecular Weight	644 aa, 72 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC060039
Gene Symbol	Kininogen 1
Gene ID (NCBI)	3827
RRID	AB_2881522
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P01042
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.