Importin Alpha 5 Polyclonal antibody

• Featured Product
• KD/KO Validated

Importin Alpha 5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (1)

Cat no : 18137-1-AP

Synonyms

IMA5, KPNA1, IPOA5, Impα5, Importin subunit alpha-5, N-terminally processed

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Validation Data Gallery

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  WB analysis using 18137-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18137-1-AP (importin α5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18137-1-AP

  WB result of Importin Alpha 5 antibody (18137-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Importin Alpha 5 transfected HepG2 cells.

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  IP experiment of HEK-293 using 18137-1-AP

  IP result of anti-Importin Alpha 5 (IP:18137-1-AP, 4ug; Detection:18137-1-AP 1:1000) with HEK-293 cells lysate 1480ug.

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  IHC staining of human testis using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18137-1-AP (importin α5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 18137-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18137-1-AP (importin α5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 18137-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Importin Alpha 5 antibody (18137-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18137-1-AP

  Immunofluorescent analysis of MCF-7 cells, using KPNA1 antibody 18137-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human testis tissue, human kidney tissue, human placenta tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

18137-1-AP targets Importin Alpha 5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Importin Alpha 5 fusion protein Ag12751
• Full Name: karyopherin alpha 1 (importin alpha 5)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC002374
• Gene Symbol: Importin Alpha 5
• Gene ID (NCBI): 3836
• RRID: AB_2133553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.

Publications

Species	Application	Title
human,mouse	WB	Nat Immunol; Tumor-derived exosomes antagonize innate antiviral immunity.; Authors - Liang Gao
mouse	WB,IF	Acta Pharm Sin B; Activated PKB/GSK-3β synergizes with PKC-δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I.; Authors - Hao Zeng
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
human	WB	Nat Commun; CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.; Authors - Youzhou Sang
mouse	WB	Proc Natl Acad Sci U S A; Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.; Authors - Takahiro Kawasaki
mouse	WB	PLoS Biol; The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination.; Authors - Benjamin M Laitman ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

María (Verified Customer) (02-08-2022)

Works for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RPE-1/MSC