KRCC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

KRCC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16916-1-AP

Synonyms

Lysine-rich coiled-coil protein 1, Cryptogenic hepatitis-binding protein 2, CHBP2, BM-044, BM-003

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Validation Data Gallery

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  WB analysis using 16916-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16916-1-AP (KRCC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 16916-1-AP

  WB result of KRCC1 antibody (16916-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KRCC1 transfected MCF-7 cells.

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  IF Staining of HepG2 using 16916-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16916-1-AP (KRCC1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

16916-1-AP targets KRCC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRCC1 fusion protein Ag10157
• Full Name: lysine-rich coiled-coil 1
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC107580
• Gene Symbol: KRCC1
• Gene ID (NCBI): 51315
• RRID: AB_2878331
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	FASEB J; KRCC1: A potential therapeutic target in ovarian cancer.; Authors - Shailendra Kumar Dhar Dwivedi
human	WB,IF	Nucleic Acids Res; KRCC1, a modulator of the DNA damage response; Authors - Fiifi Neizer-Ashun ; KD Validated