Cytokeratin 15 Polyclonal antibody

Cytokeratin 15 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10137-1-AP

Synonyms

CK 15, CK15, Cytokeratin 15, K15, K1CO, keratin 15, KRT15, KRTB



Tested Applications

Positive WB detected inA431 cells, mouse thymus tissue, HeLa cells, PC-3 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

10137-1-AP targets Cytokeratin 15 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 15 fusion protein Ag0185
Full Name keratin 15
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002641
Gene symbol KRT15
Gene ID (NCBI) 3866
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IHC protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
IP protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
FC protocol for Cytokeratin 15 antibody 10137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Stem Cells

LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.

Authors - R Sartaj
humanIF

J Invest Dermatol

GW bodies: cytoplasmic compartments in normal human skin.

Authors - Zee Jennifer M JM
humanWB

Carcinogenesis

Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.

Authors - Gautam Adhikary
mouseIF

Sci Rep

Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.

Authors - R Sartaj
ratWB,IF

Front Med (Lausanne)

Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells.

Authors - Shaoheng Xiong
humanIF

PLoS One

Identification of a population of epidermal squamous cell carcinoma cells with enhanced potential for tumor formation.

Authors - Gautam Adhikary