Validation Data Gallery
|Positive WB detected in
|A431 cells, mouse thymus tissue, HeLa cells, PC-3 cells
|Positive IP detected in
|Positive IHC detected in
|human cervical cancer tissue, human oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:400-1:1600
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10137-1-AP targets Cytokeratin 15 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Cytokeratin 15 fusion protein Ag0185
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 15 is a type I cytokeratin. It is found in some progenitor basal cells within complex epithelia.
A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring
LIM Homeobox Domain 2 (Lhx2) is required for corneal epithelial homeostasis.
J Invest Dermatol
GW bodies: cytoplasmic compartments in normal human skin.
Survival Of Skin Cancer Stem Cells Requires The Ezh2 Polycomb Group Protein.
Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.
Front Med (Lausanne)
Metformin Promotes Mechanical Stretch-Induced Skin Regeneration by Improving the Proliferative Activity of Skin-Derived Stem Cells.