KRT34 Polyclonal antibody

KRT34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20449-1-AP

Synonyms

keratin 34, KRT 34, Keratin-34, keratin34

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Validation Data Gallery

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  WB analysis of HeLa using 20449-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20449-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20449-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 20449-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 20449-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20449-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20449-1-AP (KRT34 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung squamous cell carcinoma using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung squamous cell carcinoma using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 20449-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 20449-1-AP (KRT34 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 20449-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 20449-1-AP (KRT34 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, A431 cells, HepG2 cells, L02 cells, mouse skin tissue
• Positive IHC detected in: human skin cancer tissue, human lung squamous cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20449-1-AP targets KRT34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRT34 fusion protein Ag14295
• Full Name: keratin 34
• Calculated Molecular Weight: 436 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC041070
• Gene Symbol: KRT34
• Gene ID (NCBI): 3885
• RRID: AB_10700000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.