Cytokeratin 6A-specific Polyclonal antibody

Cytokeratin 6A-specific Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 16853-1-AP

Synonyms

CK 6A, CK 6D, CK6A, CK6C, CK6D, Cytokeratin 6A, Cytokeratin 6D, Hom s 5, K6A, K6C, K6D, keratin 6A, KRT conserve, KRT6, KRT6A, KRT6C, KRT6D, Type II keratin Kb6

---

Validation Data Gallery

• 

  WB analysis of A431 using 16853-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16853-1-AP (Cytokeratin 6A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16853-1-AP

  IP Result of anti-Cytokeratin 6A-specific (IP:16853-1-AP, 4ug; Detection:16853-1-AP 1:300) with HeLa cells lysate 520ug.

---

Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, HeLa cells, human saliva tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Product Information

16853-1-AP targets Cytokeratin 6A-specific in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 6A
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 56 kDa
• GenBank accession number: NM_005554
• Gene symbol: KRT6A
• Gene ID (NCBI): 3853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence. This antibody is against all three isoforms of keratin 6, but it does not bind other keratins.