Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22208-1-AP

Synonyms

CK 7, CK7, Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue, human kidney tissue, human breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22208-1-AP is Cytokeratin 7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag17528
Full Name keratin 7
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC002700
Gene symbol KRT7
Gene ID (NCBI) 3855
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
IHC protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
IF protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
FC protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
Standard Protocols
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