Cytokeratin 7 Polyclonal antibody
Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 22208-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells |
Positive IHC detected in | human lung cancer tissue, human pancreas tissue, human kidney tissue, human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells, HeLa cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
Product Information
22208-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 7 fusion protein Ag17528 |
Full Name | keratin 7 |
Calculated Molecular Weight | 469 aa, 51 kDa |
Observed Molecular Weight | 51 kDa |
GenBank Accession Number | BC002700 |
Gene Symbol | KRT7 |
Gene ID (NCBI) | 3855 |
RRID | AB_2879030 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 7 antibody 22208-1-AP | Download protocol |
IHC protocol for Cytokeratin 7 antibody 22208-1-AP | Download protocol |
IF protocol for Cytokeratin 7 antibody 22208-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Ginekol Pol Expression profiles of human somatic mesenchymal stem cells derived from fresh endometrium, ectopic-endometrium and umbilical cord |