Cytokeratin 8 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cytokeratin 8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10384-1-AP

Synonyms

KRT8, Cytokeratin-8, Cytokeratin,Keratin, CK-8, CK8

---

Validation Data Gallery

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10384-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10384-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10384-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10384-1-AP

  IP result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded normal colon slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 10384-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of human breast cancer using 10384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 10384-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 8 antibody (10384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

• 

  FC experiment of MCF-7 using 10384-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Cytokeratin 8 Polyclonal antibody (10384-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HeLa cells, HaCaT cells, COLO 320 cells, A431 cells, A549 cells, MCF-7 cells, mouse skin tissue, rat skin tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue, human breast cancer tissue, human colon tissue, human liver tissue, human normal colon, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: A431 cells, HeLa cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:40000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

10384-1-AP targets Cytokeratin 8 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, goat, dog
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 8 fusion protein Ag0488
• Full Name: keratin 8
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC000654
• Gene Symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• RRID: AB_10638912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Publications

Species	Application	Title
human	WB,IF,IP	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network; Authors - Archan Chakraborty ; KD Validated
mouse	WB,IF	Front Cell Dev Biol; ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.; Authors - Shuai Yu
human	WB,IF	J Cell Sci; SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.; Authors - Archan Chakraborty ; KD Validated
human, goat, dog, pig	IHC	Anat Rec (Hoboken); Comparative study of the external auditory canal in humans and large mammals.; Authors - Jun Wang
human	WB,IF	Oncotarget; Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.; Authors - Sike Hu
mouse	IHC	Oncoscience; The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.; Authors - Dhanushka Hewa Bostanthirige

Related Products

Cytokeratin 18 Polyclonal antibody

10830-1-AP

Cytokeratin 18 Monoclonal antibody

66187-1-Ig

Cytokeratin 19 Polyclonal antibody

10712-1-AP

Cytokeratin 19 Monoclonal antibody

60187-1-Ig

Vimentin Polyclonal antibody

10366-1-AP

ERK1/2 Polyclonal antibody

11257-1-AP

uPA/Urokinase Polyclonal antibody

17968-1-AP

Cytokeratin 7-specific Polyclonal antibody

17513-1-AP

Cytokeratin 14 Polyclonal antibody

10143-1-AP

TRAIL Polyclonal antibody

17235-1-AP