Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10384-1-AP

Synonyms

CARD2, CK 8, CK8, CYK8, Cytokeratin 8, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8



Tested Applications

Positive WB detected inMCF7 cells, A431 cells, A549 cells, COLO 320 cells, HeLa cells, rat skin tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, HepG2 cells, A431 cells, MCF-7 cells
Positive FC detected inHepG2 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 10384-1-AP is Cytokeratin 8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, goat, human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 8 fusion protein Ag0488
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC000654
Gene symbol KRT8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
IHC protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
IF protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
IP protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
FC protocol for Cytokeratin 8 antibody 10384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Oncotarget

Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.

Authors - Sike Hu
mouseIHC

Oncoscience

The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.

Authors - Dhanushka Hewa Bostanthirige
human, goat, dog, pigIHC

Anat Rec (Hoboken)

Comparative study of the external auditory canal in humans and large mammals.

Authors - Jun Wang
humanWB,IF

J Cell Sci

SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.

Authors - Archan Chakraborty
  • KD Validated