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- KD/KO Validated
Cytokeratin 8 Polyclonal antibody
Cytokeratin 8 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 10384-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HaCaT cells, COLO 320 cells, A431 cells, A549 cells, MCF-7 cells, mouse skin tissue, rat skin tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human tonsillitis tissue, human colon tissue, human appendicitis tissue, human breast cancer tissue, human renal cell carcinoma tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells, HepG2 cells, human breast cancer tissue, HeLa cells |
Positive FC detected in | HepG2 cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:40000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 5 publications below |
IHC | See 4 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
Product Information
10384-1-AP targets Cytokeratin 8 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, dog, goat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cytokeratin 8 fusion protein Ag0488 |
Full Name | keratin 8 |
Calculated molecular weight | 54 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | BC000654 |
Gene symbol | KRT8 |
Gene ID (NCBI) | 3856 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 8 antibody 10384-1-AP | Download protocol |
IHC protocol for Cytokeratin 8 antibody 10384-1-AP | Download protocol |
IF protocol for Cytokeratin 8 antibody 10384-1-AP | Download protocol |
IP protocol for Cytokeratin 8 antibody 10384-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Sci SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network
| ||
Front Cell Dev Biol ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2. | ||
J Cell Sci SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.
| ||
Anat Rec (Hoboken) Comparative study of the external auditory canal in humans and large mammals. | ||
Oncotarget Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation. | ||
Oncoscience The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate. |