Validation Data Gallery
|Positive WB detected in||MCF7 cells, A431 cells, A549 cells, COLO 320 cells, HeLa cells, rat skin tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue, HepG2 cells, A431 cells, MCF-7 cells|
|Positive FC detected in||HepG2 cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 10384-1-AP is Cytokeratin 8 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||dog, goat, human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 8 fusion protein Ag0488|
|Full Name||keratin 8|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||52 kDa|
|GenBank accession number||BC000654|
|Gene ID (NCBI)||3856|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.
|Product Specific Protocols|
|WB protocol for Cytokeratin 8 antibody 10384-1-AP||Download protocol|
|IHC protocol for Cytokeratin 8 antibody 10384-1-AP||Download protocol|
|IF protocol for Cytokeratin 8 antibody 10384-1-AP||Download protocol|
|IP protocol for Cytokeratin 8 antibody 10384-1-AP||Download protocol|
|FC protocol for Cytokeratin 8 antibody 10384-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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