Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human ovary tumor tissue, rat adrenal gland tissue, human colon tissue, human bowen disease tissue, mouse brain tissue, rat small intestine tissue, human ovary cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19841-1-AP targets KTN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13854 Product name: Recombinant human KTN1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 951-1306 aa of BC117132 Sequence: KSQIEQLKQQNYQQASSFPPHEELLKVISEREKEISGLWNELDSLKDAVEHQRKKNNDLREKNWEAMEALASTEKMLQDKVNKTSKERQQQVEAVELEAKEVLKKLFPKVSVPSNLSYGEWLHGFEKKAKECMAGTSGSEEVKVLEHKLKEADEMHTLLQLECEKYKSVLAETEGILQKLQRSVEQEENKWKVKVDESHKTIKQMQSSFTSSEQELERLRSENKDIENLRREREHLEMELEKAEMERSTYVTEVRELKAQLNETLTKLRTEQNERQKVAGDLHKAQQSLELIQSKIVKAAGDTTVIENSDVSPETESSEKETMSVSLNQTVTQLQQLLQAVNQQLTKEKEHYQVLE Predict reactive species |
| Full Name | kinectin 1 (kinesin receptor) |
| Calculated Molecular Weight | 1357 aa, 156 kDa |
| Observed Molecular Weight | 156 kDa |
| GenBank Accession Number | BC117132 |
| Gene Symbol | KTN1 |
| Gene ID (NCBI) | 3895 |
| RRID | AB_10642436 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UP2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KTN1 antibody 19841-1-AP | Download protocol |
| IHC protocol for KTN1 antibody 19841-1-AP | Download protocol |
| IP protocol for KTN1 antibody 19841-1-AP | Download protocol |
| WB protocol for KTN1 antibody 19841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KTN1 Polyclonal antibody (Cat# 19841-1-AP) has 15 citations spanning journals including Nature, Science, Cell Research, Nature Communications, Science Translational Medicine, Current Biology, EMBO Reports, and others. Research fields covered include ER remodeling, organelle distribution, cardiomyopathy, DNA damage-induced apoptosis, nuclear pore assembly, Huntington's disease, gastric cancer, mitotic progression, selective autophagy, bladder cancer, immune synapse biology, and ER sheet organization. Applications cited are primarily WB, IF, IHC, CoIP, and IP.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy. | ||
Nature ER proteins decipher the tubulin code to regulate organelle distribution
| ||
Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function
| ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Nat Commun RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion. | ||
Sci Transl Med CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease. |
Reviews
What customers say
The single review for this antibody reports a positive experience with Western blot. The user tested it at a 1:1000 dilution on mouse heart tissue and found it performed well, awarding a 5-star rating. Overall, the feedback indicates reliable WB performance for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Siting (Verified Customer) (01-28-2020) | This antibody worked well with western blot.
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