KTN1 Recombinant antibody

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  WB analysis using 85681-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85681-4-RR (KTN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 85681-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85681-4-RR (KTN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 85681-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85681-4-RR (KTN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85681-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (85681-4-RR, Clone: 243067A11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

KTN1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85681-4-RR

Clone No.243067A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CG-1 antigen, KIAA0004, KTN 1

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells, A549 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85681-4-RR targets KTN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KTN1 fusion protein Ag13854
• Full Name: kinectin 1 (kinesin receptor)
• Calculated Molecular Weight: 1357 aa, 156 kDa
• Observed Molecular Weight: 156 kDa
• GenBank Accession Number: BC117132
• Gene Symbol: KTN1
• Gene ID (NCBI): 3895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86UP2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.