Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11796-1-AP targets KYNU in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2365 Product name: Recombinant human KYNU protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC000879 Sequence: MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPARSEFFN Predict reactive species |
| Full Name | kynureninase (L-kynurenine hydrolase) |
| Calculated Molecular Weight | 465aa,52 kDa; 307aa,35 kDa |
| Observed Molecular Weight | 45-52 kDa |
| GenBank Accession Number | BC000879 |
| Gene Symbol | KYNU |
| Gene ID (NCBI) | 8942 |
| RRID | AB_2133050 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16719 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755). This protein is a 95-kD homodimer predominantly located in the cytoplasm(PMID:9180257).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KYNU antibody 11796-1-AP | Download protocol |
| IHC protocol for KYNU antibody 11796-1-AP | Download protocol |
| WB protocol for KYNU antibody 11796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Cell Metab Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury | ||
Nat Commun Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma | ||
Theranostics GPX4 knockdown suppresses M2 macrophage polarization in gastric cancer by modulating kynurenine metabolism | ||
Adv Sci (Weinh) Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth
| ||
Int J Biol Sci Immune Cell Infiltration and Kynurenine Pathway Activation Define Early Injury and Progression in Diabetic Nephropathy. | ||
Phytomedicine Kuntai capsule ameliorates PCOS by reversing tryptophan metabolic shift and aberrant nuclear AHR activation: a multi-omics investigation. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The reviewer used it for Western Blot on HeLa cells at a 1:1000 dilution, noting that it works well when dissolved in 5% BSA in TBST. Overall, the feedback is positive, confirming reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hala (Verified Customer) (03-29-2022) | it works good in BSA 5% dissolved in TBST
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