Ki-67 Recombinant monoclonal antibody

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• Ki-67 Recombinant monoclonal antibody (84432-1-RR)

Ki67 antibodies are widely used as markers for cell proliferation, enabling the detection and quantification of actively dividing cells in tissue samples for research and diagnostic applications, especially in cancer pathology. Proteintech has an extensive range of Ki-67 antibodies validated for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA, Cell treatment and more.

Cat No. 84432-1-RR Copied!

Clone No. 241768E2

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ki 67, Ki67, Mki67, 241768E2, Proliferation related Ki 67 antigen

• 

  WB analysis of NIH/3T3 using 84432-1-RR

  NIH/3T3 cells were subjected to SDS page followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of C2C12 using 84432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of C2C12 using 84432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of NIH/3T3 using 84432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: mouse spleen tissue, rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84432-1-RR targets Ki-67 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1548

  Product name: Recombinant Mouse Ki-67 protein (N-rFc)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 1816-2163 aa of NM_001081117.2

  Sequence: DETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTP

• Full Name: antigen identified by monoclonal antibody Ki 67
• Calculated Molecular Weight: 351kDa
• Observed Molecular Weight: 350 kDa
• GenBank Accession Number: NM_001081117.2
• Gene Symbol: ki67
• Gene ID (NCBI): 17345
• RRID: AB_3671959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: E9PVX6-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Publications

What published studies show

Ki-67 Recombinant monoclonal antibody (241768E2), catalog number 84432-1-RR, has accumulated 18 citations across journals including Nature Communications, Small, Acta Biomaterialia, Materials Today Bio, FASEB Journal, Scientific Reports, and International Immunopharmacology. Associated research fields span bone regeneration, pulmonary hypertension, CAR-T cell immunotherapy, wound healing, cartilage regeneration, oncology (breast, liver, colorectal cancer), fibrosis (hepatic, renal), cardiac injury, psoriasis, and endometrial repair.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IF	Nat Commun; Directed rescue strategy for enhanced implant osteointegration in aged rats.; Authors - Xuan Li
		J Nanobiotechnology; Hypoxia-injured pulmonary vascular targeting and microenvironment-responsive nanoparticle to treat hypoxic pulmonary hypertension.; Authors - Hongyan Wu
mouse	WB,IF	Small; IL-21 Loading CaMnCO3 Vitality Backpacks Boost CAR-T Cell Synergistic Immunotherapy; Authors - Baihui Wang
mouse	IHC	Mater Today Bio; A smart CO-releasing MnSiO3-based hydrogel enhances diabetic wound healing through NIR-triggered antibacterial, anti-inflammatory, and pro-regenerative mechanisms; Authors - Yukun Liu
rat	IF	Acta Biomater; Supramolecular dynamic hydrogels loaded with bevacizumab nanoparticles promote hyaline cartilage regeneration by inhibiting angiogenesis.; Authors - Aikang Li
		Life Sci; Fecal microbiota transplantation promotes hair growth through gut microbiome and metabolic regulation; Authors - Yangyang Tian