FcZero-rAb® Biotin Anti-Mouse Ki-67 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using Biotin-FcA98224

  1x10^6 untreated or Con A-treated mouse splenocytes were intracellularly stained with 0.25 ug Biotin Anti-Mouse Ki-67 Rabbit RecAb (Biotin-FcA98224, Clone: 241768E2) and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of mouse splenocytes using Biotin-FcA98224

  1x10^6 Con A-treated mouse splenocytes were intracellularly stained with 0.25 ug Biotin Anti-Mouse Ki-67 Rabbit RecAb (Biotin-FcA98224, Clone: 241768E2) (red) or CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) (blue), and Streptavidin-PE Conjugate (PE-PF00030). 1x10^6 untreated mouse splenocytes were intracellularly stained with 0.25 ug Biotin Anti-Mouse Ki-67 Rabbit RecAb (Biotin-FcA98224, Clone: 241768E2) (black, dashed) and Streptavidin-PE Conjugate (PE-PF00030). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

Ki-67 FcZero-rAb^® Recombinant Antibody for FC (Intra), ELISA

Cat No. Biotin-FcA98224

Clone No.241768E2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra), ELISA

Synonyms

Ki 67, Ki67, Mki67, Proliferation related Ki 67 antigen

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Tested Applications

• Positive FC (Intra) detected in: Con A treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98224 targets Ki-67 in FC (Intra), ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1548

  Product name: Recombinant Mouse Ki-67 protein (N-rFc)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 1816-2163 aa of NM_001081117.2

  Sequence: DETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTP

• Full Name: antigen identified by monoclonal antibody Ki 67
• Calculated Molecular Weight: 351kDa
• GenBank Accession Number: NM_001081117.2
• Gene Symbol: ki67
• Gene ID (NCBI): 17345
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: E9PVX6-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.