LACTB Polyclonal antibody

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  WB analysis of HepG2 using 18195-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18195-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18195-1-AP (LACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18195-1-AP (LACTB antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse liver using 18195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using LACTB antibody (18195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

LACTB Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 18195-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

EC:3.4.-.-, G24, lactamase, beta, MRPL56, Serine beta-lactamase-like protein LACTB, mitochondrial

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Tested Applications

• Positive WB detected in: HepG2 cells, human heart tissue, HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

18195-1-AP targets LACTB in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12233

  Product name: Recombinant human LACTB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 73-373 aa of BC067288

  Sequence: DPEASPLAEPPQEQSLAPWSPQTPAPPCSRCFARAIESSRDLLHRIKDEVGAPGIVVGVSVDGKEVWSEGLGYADVENRVPCKPETVMRIASISKSLTMVALAKLWEAGKLDLDIPVQHYVPEFPEKEYEGEKVSVTTRLLISHLSGIRHYEKDIKKVKEEKAYKALKMMKENVAFEQEKEGKSNEKNDFTKFKTEQENEAKCRNSKPGKKKNDFEQGELYLREKFENSIESLRLFKNDPLFFKPGSQFLYSTFGYTLLAAIVERASGCKYLDYMQKIFHDLDMLTTVQEENEPVIYNRAR

• Full Name: lactamase, beta
• Calculated Molecular Weight: 547 aa, 61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC067288
• Gene Symbol: LACTB
• Gene ID (NCBI): 114294
• RRID: AB_2280998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P83111
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LACTB is also named as MRPL56,which is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form. This antibody is specific to LACTB.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,IF	Nature; LACTB is a tumour suppressor that modulates lipid metabolism and cell state.; Authors - Zuzana Keckesova
human	WB,IHC,IF	Oncogene; LACTB, a novel epigenetic silenced tumor suppressor, inhibits colorectal cancer progression by attenuating MDM2-mediated p53 ubiquitination and degradation.; Authors - Kaixuan Zeng ; KO Validated
Human	IHC	PLoS One; LACTB mRNA expression is increased in pancreatic adenocarcinoma and high expression indicates a poor prognosis.; Authors - Jian Xie
human	IHC	Cancer Biother Radiopharm; Clinical Significance of β-Lactamase Expression in Colorectal Cancer.; Authors - Shaofei Zhou
human	WB	Exp Ther Med; LACTB suppresses carcinogenesis in lung cancer and regulates the EMT pathway.; Authors - Yihui Xu ; KD Validated
human	WB,IF	Apoptosis; LACTB induces cancer cell death through the activation of the intrinsic caspase-independent pathway in breast cancer; Authors - Juan M Gonzalez-Morena