WB result of LACTB2 antibody (16783-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.
WB analysis using 16783-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16783-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
PC-3 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 16783-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Y79 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 16783-1-AP
IP result of anti-LACTB2 (IP:16783-1-AP, 3ug; Detection:16783-1-AP 1:700) with A549 cells lysate 520ug.
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MCF-7 cells, PC-3 cells, Y79 cells, A549 cells, HeLa cells
Positive IP detected in
A549 cells
Positive IHC detected in
human cervix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:8000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
16783-1-AP targets LACTB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
WB result of LACTB2 antibody (16783-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.
WB analysis using 16783-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 16783-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 16783-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 16783-1-AP (LACTB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human cervix using 16783-1-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervix using 16783-1-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 16783-1-AP (LACTB2 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of A549 using 16783-1-AP
IP result of anti-LACTB2 (IP:16783-1-AP, 3ug; Detection:16783-1-AP 1:700) with A549 cells lysate 520ug.
IF/ICC Figures
IF Staining of HepG2 using 16783-1-AP
Immunofluorescent analysis of HepG2 cells, using LACTB2 antibody 16783-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
LACTB2 Polyclonal antibody
Catalog Number
16783-1-AP
Citations
-
Dilutions
WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
