LAD1 Polyclonal antibody

LAD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16136-1-AP

Synonyms

LAD, Lad 1, LAD1, LadA, ladinin 1, Linear IgA disease antigen

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Validation Data Gallery

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  WB analysis using 16136-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16136-1-AP (LAD1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 16136-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16136-1-AP (LAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16136-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16136-1-AP (LAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16136-1-AP targets LAD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAD1 fusion protein Ag9159
• Full Name: ladinin 1
• Calculated Molecular Weight: 517 aa, 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC009742
• Gene Symbol: LAD1
• Gene ID (NCBI): 3898
• RRID: AB_2081705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ladinin-1, also named as LADA, or LAD1, is an anchoring filament protein which is a component of the basement membrane zone. It is a novel component of the basement membranes and may function in contributing to the stability of the association of the epithelial layers with the underlying mesenchyme. Human ladinin is an autoantigen associated with linear IgA disease. It is a secreted protein. Immunohistochemical staining of 16136-1-AP shows strong extracellular matrixc positivity in human testis. The predicted MW of this protein is 57 kDa.

Publications

Species	Application	Title
human	WB,IHC,IF	J Transl Med; LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer; Authors - Yingming Jiang