LAD1 Polyclonal antibody

LAD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16136-1-AP

Synonyms

LAD, Lad 1, LAD1, LadA, ladinin 1, Linear IgA disease antigen



Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

16136-1-AP targets LAD1 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAD1 fusion protein Ag9159
Full Name ladinin 1
Calculated molecular weight 517 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC009742
Gene symbol LAD1
Gene ID (NCBI) 3898
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ladinin-1, also named as LADA, or LAD1, is an anchoring filament protein which is a component of the basement membrane zone. It is a novel component of the basement membranes and may function in contributing to the stability of the association of the epithelial layers with the underlying mesenchyme. Human ladinin is an autoantigen associated with linear IgA disease. It is a secreted protein. Immunohistochemical staining of 16136-1-AP shows strong extracellular matrixc positivity in human testis. The predicted MW of this protein is 57 kDa.

Protocols

Product Specific Protocols
WB protocol for LAD1 antibody 16136-1-APDownload protocol
IHC protocol for LAD1 antibody 16136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols