Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98167-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98167-1-RR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone:241294E11) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98167-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98167-1-RR against Mouse LAG-3/CD223 were performed. The affinity constant is 0.636 nM.

LAG-3/CD223 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98167-1-RR

Clone No.241294E11

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC

Synonyms

CD223, LAG-3, Ly66, Lag3, LAG 3

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

98167-1-RR targets LAG-3/CD223 in WB, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57kDa
• GenBank Accession Number: NM_008479.2
• Gene Symbol: CD223
• Gene ID (NCBI): 16768
• RRID: AB_3672310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q61790
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Immunol; Anti-tumor effectiveness of a novel bispecific antibody that blocks both PD-L1 and LAG-3; Authors - Chenxing Zhang