CoraLux Violet 510 Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using CLV510-98167

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 5 ul CoraLite®500 Anti-Mouse LAG-3/CD223 Rabbit RecAb (CLV510-98167, Clone: 241294E11) and 5 ul CoraLite® Plus 488 Anti-Mouse CD3 (17A2) (CL488-65077, Clone: 17A2). Cells were not fixed.

LAG-3/CD223 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CLV510-98167

Clone No.241294E11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD223, LAG-3, 241294E11, LAG 3, Lag3

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CLV510-98167 targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LAG-3/CD223 fusion protein Eg1220
• Full Name: lymphocyte-activation gene 3
• Calculated Molecular Weight: 57kDa
• GenBank Accession Number: NM_008479.2
• Gene Symbol: CD223
• Gene ID (NCBI): 16768
• Conjugate: CoraLux Violet 510 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 410 nm / 501 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q61790
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).