FcZero-rAb® Biotin Anti-Human LAIR1 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human LAIR1 Rabbit Recombinant Antibody (Biotin-FcA98326)

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  FC experiment of human PBMCs using Biotin-FcA98326

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human LAIR1 Rabbit RecAb (Biotin-FcA98326, Clone: 241805G2) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and PE Streptavidin (Cat.No. PF00021). Cells were co-stained with FcZero-rAb™ APC Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using Biotin-FcA98326

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human LAIR1 Rabbit RecAb (Biotin-FcA98326, Clone: 241805G2) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin (Cat.No. PF00021). Cells were not fixed. Lymphocytes were gated.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

LAIR1 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98326

Clone No.241805G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

CD305, hLAIR1, LAIR 1, LAIR-1, Leukocyte-associated immunoglobulin-like receptor 1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98326 targets LAIR1 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2329

  Product name: Recombinant Human LAIR1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 22-163 aa of BC027899

  Sequence: QEEDLPRPSISAEPGTVIPLGSHVTFVCRGPVGVQTFRLERDSRSTYNDTEDVSQASPSESEARFRIDSVREGNAGLYRCIYYKPPKWSEQSDYLELLVKESSGGPDSPDTEPGSSAGPTQRPSDNSHNEHAPASQGLKAEH

• Full Name: leukocyte-associated immunoglobulin-like receptor 1
• Calculated Molecular Weight: 287 aa, 31 kDa
• GenBank Accession Number: BC027899
• Gene Symbol: LAIR1
• Gene ID (NCBI): 3903
• RRID: AB_3747031
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6GTX8
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).