LAMA4 (Isoform 3) Polyclonal antibody

LAMA4 (Isoform 3) Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10465-1-AP

Synonyms

LAMA4 (Isoform 3), Laminin 14 subunit alpha, Laminin 8 subunit alpha, Laminin 9 subunit alpha, Laminin subunit alpha 4, laminin, alpha 4



Tested Applications

Positive WB detected inhuman heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10465-1-AP targets LAMA4 (Isoform 3) in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMA4 (Isoform 3) fusion protein Ag0736
Full Name laminin, alpha 4
Calculated molecular weight 13 kDa
Observed molecular weight 11-13 kDa
GenBank accession numberBC004241
Gene symbol LAMA4
Gene ID (NCBI) 3910
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.

Protocols

Product Specific Protocols
WB protocol for LAMA4 (Isoform 3) antibody 10465-1-APDownload protocol
IHC protocol for LAMA4 (Isoform 3) antibody 10465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Cell Int

MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression.

Authors - Zhi-Xue Yang
humanIHC

J Neurosci Res

Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone.

Authors - Xu Hongmin H
humanIHC

Neuromuscul Disord

Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis.

Authors - Doppler K K
mouseIHC

Am J Physiol Heart Circ Physiol

Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.

Authors - Li Lin
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
humanIHC

Arthritis Rheum

PMID: 21337318

Authors - Gutierrez-Roelens I I