Laminin beta 1 Polyclonal antibody

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  WB analysis of NIH/3T3 using 23498-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23498-1-AP (LAMB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23498-1-AP (Laminin beta 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 23498-1-AP

  IP result of anti-Laminin beta 1 (IP:23498-1-AP, 4ug; Detection:23498-1-AP 1:2000) with NIH/3T3 cells lysate 1920 ug.

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  IF Staining of HepG2 using 23498-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Laminin beta 1 antibody (23498-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Laminin beta 1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 23498-1-AP

Publications(40)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LAMB1, CLM, Laminin 1 subunit beta, Laminin 10 subunit beta, Laminin 12 subunit beta

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Jurkat cells
• Positive IP detected in: NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 20 publications below

Product Information

23498-1-AP targets Laminin beta 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19182

  Product name: Recombinant human LAMB1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1437-1786 aa of BC113455

  Sequence: AHNAWQKAMDLDQDVLSALAEVEQLSKMVSEAKLRADEAKQSAEDILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAEDVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLLKDISQKVAVYSTCL

• Full Name: laminin, beta 1
• Calculated Molecular Weight: 1786 aa, 198 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC113455
• Gene Symbol: Laminin beta 1
• Gene ID (NCBI): 3912
• RRID: AB_2879288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07942
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Acta Pharm Sin B; A novel PGAM5 inhibitor LFHP-1c protects blood-brain barrier integrity in ischemic stroke.; Authors - Chenglong Gao
mouse	IF	ACS Appl Mater Interfaces; Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.; Authors - Yi Li
human	WB	Cell Rep; Stub1 maintains proteostasis of master transcription factors in embryonic stem cells.; Authors - Md Mahfuz Al Mamun
mouse	IF	J Neuroinflammation; SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.; Authors - Ziyu Li
rat	WB,IF	Brain Pathol; Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.; Authors - Hongjin Wang
human	WB	Angiogenesis; SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization.; Authors - Jia-Xing Sun