LAMC1 Polyclonal antibody

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  WB analysis of DU 145 using 20928-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20928-1-AP

  WB result of LAMC1 antibody (20928-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAMC1 transfected HeLa cells.

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  WB analysis of HeLa using 20928-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of DU 145 using 20928-1-AP

  IP result of anti-LAMC1 (IP:20928-1-AP, 4ug; Detection:20928-1-AP 1:1000) with DU 145 cells lysate 3600ug.

• Featured Product
• KD/KO Validated

LAMC1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 20928-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Laminin subunit gamma 1, LAMB2, LAMC 1, Laminin subunit gamma-1, Laminin-1 subunit gamma

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells
• Positive IP detected in: DU 145 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20928-1-AP targets LAMC1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14674

  Product name: Recombinant human LAMC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1260-1609 aa of BC015586

  Sequence: AGDKAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELEVKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVNDNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAVQKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP

• Full Name: laminin, gamma 1 (formerly LAMB2)
• Calculated Molecular Weight: 178 kDa
• Observed Molecular Weight: 220 kDa, 178 kDa
• GenBank Accession Number: BC015586
• Gene Symbol: LAMC1
• Gene ID (NCBI): 3915
• RRID: AB_2878767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11047
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.