LAMC2 Polyclonal antibody

LAMC2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 19698-1-AP

Synonyms

B2T, BM600, CSF, CSF 140 kDa subunit, EBR2, EBR2A, Epiligrin subunit gamma, Kalinin subunit gamma, Ladsin 140 kDa subunit, LAMB2T, LAMC2, Laminin 5 subunit gamma, Laminin B2t chain, Laminin subunit gamma 2, laminin, gamma 2, LAMNB2, Nicein subunit gamma

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Validation Data Gallery

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  WB analysis of A431 using 19698-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19698-1-AP (LAMC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

19698-1-AP targets LAMC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: laminin, gamma 2
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_005562
• Gene Symbol: LAMC2
• Gene ID (NCBI): 3918
• RRID: AB_10644139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMC2, also named as LAMB2T and LAMNB2, is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. LAMC2 binds to cells via a high affinity receptor. Laminin exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells.

Publications

Species	Application	Title
rat	WB	J Proteomics; Proteomic analysis reveals the protective effects of emodin on severe acute pancreatitis induced lung injury by inhibiting neutrophil proteases activity.; Authors - Caiming Xu
human	WB	Mol Med Rep; Laminin‑332 mediates proliferation, apoptosis, invasion, migration and epithelial‑to‑mesenchymal transition in pancreatic ductal adenocarcinoma.; Authors - Caiqun Huang
human	WB,IHC	Sci Transl Med; Connexins orchestrate progression of breast cancer metastasis to the brain by promoting FAK activation; Authors - Girieca Lorusso
human	WB,IF	J Transl Med; Endothelium-specific deletion of p62 causes organ fibrosis and cardiac dysfunction; Authors - Jing Feng
mouse	WB	iScience; Hypoxia promotes histone H3K9 lactylation to enhance LAMC2 transcription in esophageal squamous cell carcinoma; Authors - Yong Zang
human	WB	Toxicol Appl Pharmacol; The anti-cancer mechanism of Celastrol by targeting JAK2/STAT3 signaling pathway in gastric and ovarian cancer; Authors - Kang Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

Clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: ARPE-19

LAMC2 Antibody Western Blot validation (1:1000 dilution) in ARPE-19 (Cat no:19698-1-AP)