LAMP2 Monoclonal antibody

LAMP2 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgA

Reactivity

human, mouse, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2D3B9

Cat no : 66301-1-Ig

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, pig liver tissue, L02 cells, HeLa cells, HepG2 cells, HUVEC cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, 3T3-L1 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66301-1-Ig targets LAMP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgA
Class Monoclonal
Type Antibody
Immunogen LAMP2 fusion protein Ag0595
Full Name lysosomal-associated membrane protein 2
Calculated molecular weight 45 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC002965
Gene symbol LAMP2
Gene ID (NCBI) 3920
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

Protocols

Product Specific Protocols
WB protocol for LAMP2 antibody 66301-1-IgDownload protocol
IHC protocol for LAMP2 antibody 66301-1-IgDownload protocol
IF protocol for LAMP2 antibody 66301-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

Lysosomal dysfunction and autophagy blockade contribute to autophagy-related cancer suppressing peptide-induced cytotoxic death of cervical cancer cells through the AMPK/mTOR pathway.

Authors - Yang Yang
humanWB

Cell Death Dis

Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI.

Authors - Chuan'ai Chen
humanWB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
humanWB

Vet Res

Butyrate-mediated autophagy inhibition limits cytosolic Salmonella Infantis replication in the colon of pigs treated with a mixture of Lactobacillus and Bacillus.

Authors - Bingxin Chu
humanWB

Clin Transl Sci

Identification of BAG2 and Cathepsin D as Plasma Biomarkers for Parkinson's Disease.

Authors - Juhee Kang
mouseIHC

FASEB J

Glucose-induced decrease of cystathionine β-synthase mediates renal injuries.

Authors - Yanting Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Eric (Verified Customer) (03-13-2021)

I had success with some and others not. I used microglia and I did not get strong expression but in macrophages I got better bands with same dilution and treatment. Many nonspecific bands

  • Applications: Western Blot,Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Microglia/Macrophages
LAMP2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Microglia/Macrophages (Cat no:66301-1-Ig)
FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

miguel (Verified Customer) (12-11-2018)

The antibody was used with an inhouse protocol for Immunohistochemistry. The results varied from good to great for both stains and imaging.

  • Applications: Western Blot,Immunohistochemistry,
  • Cell Tissue Type: Rat Brain