|Positive WB detected in||HEK-293 cells, mouse liver tissue, pig liver tissue, L02 cells, HeLa cells, HepG2 cells, HUVEC cells, Jurkat cells|
|Positive IHC detected in||human liver cancer tissue, human liver tissue, human lung cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, 3T3-L1 cells, HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66301-1-Ig targets LAMP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, pig samples.
|Tested Reactivity||human, mouse, pig|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgA|
|Immunogen||LAMP2 fusion protein Ag0595|
|Full Name||lysosomal-associated membrane protein 2|
|Calculated molecular weight||45 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC002965|
|Gene ID (NCBI)||3920|
|Purification Method||Thiophilic affinity chromatograph|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Eric (Verified Customer) (03-13-2021)
I had success with some and others not. I used microglia and I did not get strong expression but in macrophages I got better bands with same dilution and treatment. Many nonspecific bands
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.
miguel (Verified Customer) (12-11-2018)
The antibody was used with an inhouse protocol for Immunohistochemistry. The results varied from good to great for both stains and imaging.