LANCL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LANCL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12647-1-AP

Synonyms

GPR69A, LanC like protein 1, LANCL1, p40

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Validation Data Gallery

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  WB analysis of mouse testis using 12647-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12647-1-AP (LANCL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12647-1-AP (LANCL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12647-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12647-1-AP (LANCL1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

12647-1-AP targets LANCL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LANCL1 fusion protein Ag3361
• Full Name: LanC lantibiotic synthetase component C-like 1 (bacterial)
• Calculated Molecular Weight: 399 aa, 45 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC028685
• Gene Symbol: LANCL1
• Gene ID (NCBI): 10314
• RRID: AB_10638314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.

Publications

Species	Application	Title
human	WB	Cell Death Dis; LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway.; Authors - Jianqing Wang ; KD Validated
human,mouse	WB	J Lipid Res; Lanthionine synthetase C-like protein 2 (LanCL2) is Important for Adipogenic Differentiation.; Authors - Debapriya Dutta ; KD Validated
mouse	IHC	Front Microbiol; The Endogenous Alterations of the Gut Microbiota and Feces Metabolites Alleviate Oxidative Damage in the Brain of LanCL1 Knockout Mice.; Authors - Fangxing Zhang ; KO Validated