Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, mouse heart tissue, mouse testis tissue, mouse brain tissue |
| Positive IHC detected in | human ovary cancer tissue, human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
Product Information
13708-1-AP targets LARP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, drosophila samples.
| Tested Reactivity | human, mouse, rat, drosophila |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4645 Product name: Recombinant human LARP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC033856 Sequence: MPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPTVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHSVVAGGGGGEGRKRCPSQSSSRPAAMISQPPTPPTGQPVREDAKWTSQHSNTQTLGK Predict reactive species |
| Full Name | La ribonucleoprotein domain family, member 1 |
| Calculated Molecular Weight | 1096 aa, 124 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC033856 |
| Gene Symbol | LARP1 |
| Gene ID (NCBI) | 23367 |
| RRID | AB_2296670 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PKG0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LARP1 is a widely expressed RNA-binding protein. LARP1 can participate in the cellular stress response by regulating the translation of TOP class mRNA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LARP1 antibody 13708-1-AP | Download protocol |
| IHC protocol for LARP1 antibody 13708-1-AP | Download protocol |
| WB protocol for LARP1 antibody 13708-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv The 40S-LARP1 complex reprograms the cellular translatome upon mTOR inhibition to preserve the protein synthetic capacity.
| ||
Mol Cell Proteomics A new cellular interactome of SARS-CoV-2 nucleocapsid protein and its biological implications | ||
Mol Autism Ribosome profiling in mouse hippocampus: plasticity-induced regulation and bidirectional control by TSC2 and FMRP. | ||
Mol Biotechnol Expression Profile and Prognostic Significance of Pivotal Regulators for N7-Methylguanosine Methylation in Diffuse Large B-Cell Lymphoma | ||
Mol Cell The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance | ||
Int J Biol Sci AKAP1 contributes to impaired mtDNA replication and mitochondrial dysfunction in podocytes of diabetic kidney disease |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nikolett (Verified Customer) (06-30-2025) | AB works well for IHC in a 1:100 dilution for DAB staining purposes on Human Lung Cancer tissue. I used pH6 buffer for antigen retrieval.
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