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  • KD/KO Validated

LARS Polyclonal antibody

LARS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21146-1-AP

Synonyms

hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS



Tested Applications

Positive WB detected inA549 cells, Y79 cells, HeLa cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue, human brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21146-1-AP is LARS Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LARS fusion protein Ag15684
Full Name leucyl-tRNA synthetase
Calculated molecular weight 1176 aa, 134 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC151214
Gene symbol LARS
Gene ID (NCBI) 51520
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LARS antibody 21146-1-APDownload protocol
IHC protocol for LARS antibody 21146-1-APDownload protocol
IF protocol for LARS antibody 21146-1-APDownload protocol
IP protocol for LARS antibody 21146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Authors - Guokai Yan
humanWB

PLoS One

A Genome-Wide siRNA Screen in Mammalian Cells for Regulators of S6 Phosphorylation.

Authors - Angela Papageorgiou
humanIHC

Biomed Res Int

Dickkopf 4 Alone and in Combination with Leucyl-tRNA Synthetase as a Good Prognostic Biomarker for Human Colorectal Cancer

Authors - Su-Jeong Park
humanWB,IHC

J Transl Med

Identification of LARS as an essential gene for osteosarcoma proliferation through large-Scale CRISPR-Cas9 screening database and experimental verification

Authors - Wenhao Chen
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex

Authors - Krishnendu Khan
  • KD Validated
humanWB

Nat Cell Biol

A Rag GTPase dimer code defines the regulation of mTORC1 by amino acids

Authors - Peter Gollwitzer