LAT Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82901-1-RR (same clone as 82901-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82901-1-RR (LAT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

  WB result of LAT antibody (82901-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAT transfected Jurkat cells. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LAT antibody (82901-1-RR, Clone: 2P13 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug LAT Recombinant antibody (82901-1-RR, Clone:2P13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LAT Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82901-1-PBS

Clone No.2P13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

2P13, 36 kDa phosphotyrosine adapter protein, LAT1, Linker for activation of T-cells family member 1, p36 38

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Product Information

82901-1-PBS targets LAT in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: linker for activation of T cells
• Calculated Molecular Weight: 262 aa, 28 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC011563
• Gene Symbol: LAT
• Gene ID (NCBI): 27040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43561
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.