LATS1 Polyclonal antibody

LATS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30174-1-AP

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts

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Validation Data Gallery

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  WB analysis using 30174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30174-1-AP (LATS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30174-1-AP (LATS1 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

30174-1-AP targets LATS1 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LATS1 fusion protein Ag32879
• Full Name: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC002767
• Gene Symbol: LATS1
• Gene ID (NCBI): 9113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression; Authors - Yan Xu