LBP Monoclonal antibody

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• LBP Monoclonal antibody (66181-1-Ig)

LBP Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66181-1-Ig Copied!

Clone No. 3A7E3

Publications (2)

Download datasheet

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

LBP

• 

  WB analysis of Jurkat using 66181-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66181-1-Ig (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66181-1-Ig targets LBP in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13485

  Product name: Recombinant human LBP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 128-477 aa of BC022256

  Sequence: SFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFPSRRDHPP

• Full Name: lipopolysaccharide binding protein
• Calculated Molecular Weight: 481 aa, 53 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC022256
• Gene Symbol: LBP
• Gene ID (NCBI): 3929
• RRID: AB_2881576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18428
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Publications

What published studies show

The Lipopolysaccharide Binding Protein (LBP) antibody (Cat# 66181-1-Ig) has a total of 2 citations. These appear in Nature Communications (IF 18.1) and Journal of Pediatric Surgery (IF 2.3). The associated research fields include hepatic oxidative stress and lipid droplet homeostasis regulation, as well as LBP as a diagnostic biomarker for necrotizing enterocolitis. Applications cited are Western blot, immunofluorescence, with species tested in mouse, human.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IF	Nat Commun; Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis; Authors - Qilun Zhang
human	IF	J Pediatr Surg; Lipopolysaccharide-binding protein as a biomarker in the diagnosis of necrotizing enterocolitis.; Authors - Pengjian Zou