LC3A/B Antibody

LC3A/B Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B2B5

Cat no : 66139-1-Ig

Synonyms

LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66139-1-Ig is LC3A/B Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, mouse
Cited Reactivity hamster, human, mouse, rat, swine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fusion Protein
Full Name microtubule-associated protein 1 light chain 3 alpha
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa, 16 kDa
GenBank accession numberNM_032514
Gene symbol MAP1LC3A
Gene ID (NCBI) 84557
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.

Publications

SpeciesApplicationTitle
mouseIF

Mol Neurobiol

Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy.

Authors - Chongshan Dai
ratWB

J Huazhong Univ Sci Technolog Med Sci

Rapamycin protects cardiomyocytes against anoxia/reoxygenation injury by inducing autophagy through the PI3k/Akt pathway.

Authors - Lu-Qiao Wang
humanWB,IHC

Breast Cancer (Dove Med Press)

The role of the chemokine receptor XCR1 in breast cancer cells.

Authors - Xiao Li Yang
humanWB

Mol Med Rep

Pseudolaric acid B inhibits proliferation in SW579 human thyroid squamous cell carcinoma.

Authors - Jinghua Yu

Oncol Lett

Pseudolaric acid B activates autophagy in MCF-7 human breast cancer cells to prevent cell death.

Authors - Jinghua Yu
humanWB

J Bioenerg Biomembr

Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer.

Authors - Yiming Sun