LCA5 Polyclonal antibody

LCA5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 19333-1-AP

Synonyms

C6orf152, LCA5, Leber congenital amaurosis 5, Lebercilin

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Validation Data Gallery

• 

  WB analysis of HeLa using 19333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19333-1-AP (LCA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 19333-1-AP is LCA5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCA5 fusion protein Ag5140
• Full Name: Leber congenital amaurosis 5
• Calculated Molecular Weight: 693 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC050327
• Gene Symbol: LCA5
• Gene ID (NCBI): 167691
• RRID: AB_2878576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCA5 (Leber congenital amaurosis 5), also named as C6orf152 or Lebercilin, is a 697 amino acids protein. LCA5 is widely expressed at the microtubules, centrosome, and primary cilia. Recent study showed that LCA5 was involved in the cause of congenital and early-onset retinal dystrophies (PMID: 24144451). The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Publications

Species	Application	Title
mouse		Mol Ther; Amelioration of Neurosensory Structure and Function in Animal and Cellular Models of a Congenital Blindness.; Authors - Ji Yun Song
mouse	IF	Hum Mol Genet; Disruption of the Retinitis Pigmentosa 28 gene Fam161a in mice affects photoreceptor ciliary structure and leads to progressive retinal degeneration.; Authors - Marcus Karlstetter
mouse	IF	Invest Ophthalmol Vis Sci; Treatment Potential for LCA5-Associated Leber Congenital Amaurosis.; Authors - Katherine E Uyhazi
pig	IF	J Proteomics; Tissue- and isoform-specific protein complex analysis with natively processed bait proteins.; Authors - Tina Beyer
human	WB,IF,CoIP	Sci Rep; The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system; Authors - Tamás Szaniszló
human	IF	Nat Commun; iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy; Authors - Vincent Louvel