Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19333-1-AP targets LCA5 in WB, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5140 Product name: Recombinant human LCA5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 4-283 aa of BC050327 Sequence: RAGSPGTDQEREAGKHHYSYSSDFETPQSSGRSSLVSSSPASVRRKNPKRQTSDGQVHHQAPRKPSPKGLPNRKGVRVGFRSQSLNREPLRKDTDLVTKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQYRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEISEARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQRLYH Predict reactive species |
| Full Name | Leber congenital amaurosis 5 |
| Calculated Molecular Weight | 693 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC050327 |
| Gene Symbol | LCA5 |
| Gene ID (NCBI) | 167691 |
| RRID | AB_2878576 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LCA5 (Leber congenital amaurosis 5), also named as C6orf152 or Lebercilin, is a 697 amino acids protein. LCA5 is widely expressed at the microtubules, centrosome, and primary cilia. Recent study showed that LCA5 was involved in the cause of congenital and early-onset retinal dystrophies (PMID: 24144451). The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LCA5 antibody 19333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LCA5 Polyclonal antibody (Cat# 19333-1-AP) has 8 citations published in Nature Communications, Molecular Therapy, EMBO Journal, EMBO Molecular Medicine, Investigative Ophthalmology & Visual Science, Scientific Reports, Journal of Proteomics, and Human Molecular Genetics. Research fields span retinal degenerative diseases (Leber congenital amaurosis, retinitis pigmentosa), photoreceptor cilia biology, centrosome function, gene augmentation therapy, and expansion microscopy techniques.
| Species | Application | Title |
|---|---|---|
Nat Commun iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy | ||
Mol Ther Amelioration of Neurosensory Structure and Function in Animal and Cellular Models of a Congenital Blindness. | ||
EMBO J Glutamylation imbalance impairs the molecular architecture of the photoreceptor cilium | ||
Invest Ophthalmol Vis Sci Treatment Potential for LCA5-Associated Leber Congenital Amaurosis. | ||
Sci Rep The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system |
