LCOR Polyclonal antibody

LCOR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14476-1-AP

Synonyms

MLR2, Mblk1-related protein 2, Ligand-dependent corepressor, C10orf12

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Validation Data Gallery

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  WB analysis of HeLa using 14476-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14476-1-AP (LCOR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 14476-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human endometrial cancer using 14476-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14476-1-AP (LCOR antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14476-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14476-1-AP (LCOR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14476-1-AP targets LCOR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCOR fusion protein Ag5851
• Full Name: ligand dependent nuclear receptor corepressor
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC053359
• Gene Symbol: LCOR
• Gene ID (NCBI): 84458
• RRID: AB_2136862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCOR is a transcriptional corepressor widely expressed in fetal and adult tissues that is recruited to agonist-bound nuclear receptors through a single LxxLL motif, also referred to as a nuclear receptor (NR) box [PMID:12535528]. It functions through recruitment of C-terminal binding proteins (CtBPs) and histone deacetylases (HDACs) to its N-terminal and central domains, respectively [PMID:22277651]. It is a scaffold for both histone deacetylases (HDACs) and C-terminal binding protein (CtBP) corepressors and functions by both HDAC-dependent and -independent mechanisms in a receptor-specific manner[PMID:19744932].