SLP76 Polyclonal antibody

SLP76 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12728-1-AP

Synonyms

LCP2, SLP-76 tyrosine phosphoprotein, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

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Validation Data Gallery

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  WB analysis using 12728-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12728-1-AP (SLP76 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12728-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12728-1-AP (SLP76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 12728-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12728-1-AP (SLP76 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12728-1-AP

  IP result of anti-SLP76 (IP:12728-1-AP, 4ug; Detection:12728-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human tonsillitis using 12728-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12728-1-AP (SLP76 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12728-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12728-1-AP (SLP76 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 12728-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human SLP76 (12728-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, RAW264.7
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

12728-1-AP targets SLP76 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLP76 fusion protein Ag3432
• Full Name: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 70-76 kDa
• GenBank Accession Number: BC016618
• Gene Symbol: SLP76
• Gene ID (NCBI): 3937
• RRID: AB_2136718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Oncol; Transcriptome-Based Network Analysis Unveils Eight Immune-Related Genes as Molecular Signatures in the Immunomodulatory Subtype of Triple-Negative Breast Cancer.; Authors - Jinguo Zhang
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
mouse	IHC	Front Genet; Identification of Key Genes and Potential New Biomarkers for Ovarian Aging: A Study Based on RNA-Sequencing Data.; Authors - Lingwei Ma
human	IHC	Medicine (Baltimore); Identification of key genes in salivary gland in Sjögren's syndrome complicated with Hashimoto thyroiditis: Common pathogenesis and potential diagnostic markers; Authors - Kaiyuan Zhang
human	WB	Bioorg Chem; Design, synthesis, and biological evaluation of novel HPK1 inhibitors possessing 3-cyano-quinoline moiety; Authors - Long Chen