LDHAL6A Polyclonal antibody

LDHAL6A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16221-1-AP

Synonyms

L-lactate dehydrogenase A-like 6A, LDHL2, LDHA-like protein 6A, EC:1.1.1.27

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 16221-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16221-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16221-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16221-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 16221-1-AP

  Immunofluorescent analysis of Hela cells, using LDHAL6A antibody 16221-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, human brain tissue, Jurkat cells, mouse brain tissue, mouse testis tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16221-1-AP targets LDHAL6A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LDHAL6A fusion protein Ag9273
• Full Name: lactate dehydrogenase A-like 6A
• Calculated Molecular Weight: 26 kDa, 37 kDa
• Observed Molecular Weight: 30-36 kDa
• GenBank Accession Number: BC014340
• Gene Symbol: LDHAL6A
• Gene ID (NCBI): 160287
• RRID: AB_2265629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.