LDHC-Specific Polyclonal antibody

LDHC-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19989-1-AP

Synonyms

Cancer/testis antigen 32, CT32, lactate dehydrogenase C, LDH C, LDH testis subunit, LDH X, LDH3, LDHC, LDHC-Specific, LDHX

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Validation Data Gallery

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  WB analysis of human testis using 19989-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 19989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19989-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19989-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19989-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19989-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 19989-1-AP

  IP Result of anti-LDHC-Specific (IP:19989-1-AP, 4ug; Detection:19989-1-AP 1:500) with mouse testis tissue lysate 2400ug.

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  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of mouse testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human heart using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 19989-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19989-1-AP

  Immunofluorescent analysis of Hela cells, using LDHC antibody 19989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, A549 cells, rat testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue, human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

19989-1-AP targets LDHC-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: lactate dehydrogenase C
• Calculated molecular weight: 36 kDa
• Observed molecular weight: 30-36 kDa
• GenBank accession number: NM_002301
• Gene symbol: LDHC
• Gene ID (NCBI): 3948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.; Authors - Yuping Chen
	IHC	Med Sci Monit; PMID: 31332157; Authors - Li Yang
mouse	WB,IHC	Andrology; Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs; Authors - Rie Iida-Norita