LDHC-Specific Polyclonal antibody
LDHC-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19989-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, A549 cells, rat testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | mouse testis tissue, human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IHC | See 2 publications below |
Product Information
19989-1-AP targets LDHC-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | lactate dehydrogenase C |
Calculated molecular weight | 36 kDa |
Observed molecular weight | 30-36 kDa |
GenBank accession number | NM_002301 |
Gene symbol | LDHC |
Gene ID (NCBI) | 3948 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
IHC protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
IF protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
IP protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Pharmacol Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis. | ||
Andrology Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs |