LDHC-Specific Polyclonal antibody

LDHC-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19989-1-AP


Cancer/testis antigen 32, CT32, lactate dehydrogenase C, LDH C, LDH testis subunit, LDH X, LDH3, LDHC, LDHC-Specific, LDHX

Tested Applications

Positive WB detected inhuman testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, A549 cells, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, human testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19989-1-AP targets LDHC-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lactate dehydrogenase C
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 30-36 kDa
GenBank Accession NumberNM_002301
Gene Symbol LDHC
Gene ID (NCBI) 3948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.


Product Specific Protocols
WB protocol for LDHC-Specific antibody 19989-1-APDownload protocol
IHC protocol for LDHC-Specific antibody 19989-1-APDownload protocol
IF protocol for LDHC-Specific antibody 19989-1-APDownload protocol
IP protocol for LDHC-Specific antibody 19989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Pharmacol

Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis.

Authors - Yuping Chen

Med Sci Monit

PMID: 31332157

Authors - Li Yang


Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs

Authors - Rie Iida-Norita


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Shinford (Verified Customer) (09-23-2023)

Utilizing the concentration of 1:1000, WB results are clear in kinds lung cancer cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lung cancer cell lines
LDHC-Specific Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines (Cat no:19989-1-AP)