LDHC-Specific Polyclonal antibody

LDHC-Specific Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19989-1-AP

Synonyms

Cancer/testis antigen 32, CT32, lactate dehydrogenase C, LDH C, LDH testis subunit, LDH X, LDH3, LDHC, LDHX



Tested Applications

Positive WB detected inhuman testis tissue, A549 cells, mouse lung tissue, mouse brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, human testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

19989-1-AP targets LDHC-Specific in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lactate dehydrogenase C
Calculated molecular weight 36 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberNM_002301
Gene symbol LDHC
Gene ID (NCBI) 3948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

Protocols

Product Specific Protocols
WB protocol for LDHC-Specific antibody 19989-1-APDownload protocol
IHC protocol for LDHC-Specific antibody 19989-1-APDownload protocol
IF protocol for LDHC-Specific antibody 19989-1-APDownload protocol
IP protocol for LDHC-Specific antibody 19989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Med Sci Monit

PMID: 31332157

Authors - Li Yang