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LDHD Polyclonal antibody, PBS Only (14398-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 14398-1-AP (same clone as 14398-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)
Immunofluorescent analysis of HepG2 cells, using LDHD antibody 14398-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

LDHD Polyclonal antibody, PBS Only

Name: LDHD Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14398-1-PBS

LDHD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14398-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14398-1-PBS targets LDHD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5897 Product name: Recombinant human LDHD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 159-507 aa of BC047902 Sequence: GADASLCGMAATGASGTNAVRYGTMRDNVLNLEVVLPDGRLLHTAGRGRHFRFGFWPEIPHHTAWYSPCVSLGRRKSAAGYNLTGLFVGSEGTLGLITATTLRLHPAPEATVAATCAFPSVQAAVDSTVHILQAAVPVARIEFLDEVMMDACNRYSKLNCLVAPTLFLEFHGSQQALEEQLQRTEEIVQQNGASDFSWAKEAEERSRLWTARHNAWYAALATRPGCKGYSTDVCVPISRLPEIVVQTKEDLNASGLTGSIVGHVGDGNFHCILLVNPDDAEELGRVKAFAEQLGRRALALHGTCTGEHGIGMGKRQLLQEEVGAVGVETMRQLKAVLDPQGLMNPGKVL Predict reactive species
Full Name	lactate dehydrogenase D
Calculated Molecular Weight	52 kDa
Observed Molecular Weight	45-54 kDa
GenBank Accession Number	BC047902
Gene Symbol	LDHD
Gene ID (NCBI)	197257
RRID	AB_2249831
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86WU2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.