LEFTY2 Polyclonal antibody

LEFTY2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 13991-1-AP

Synonyms

EBAF, LEFTA, LEFTY2, LEFTYA, Protein lefty 2, Protein lefty A, TGF beta 4, TGFB4



Tested Applications

Positive WB detected inHeLa cells, mouse uterus tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13991-1-AP targets LEFTY2 in WB, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LEFTY2 fusion protein Ag5073
Full Name left-right determination factor 2
Calculated Molecular Weight 366 aa, 41 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC035718
Gene Symbol LEFTY2
Gene ID (NCBI) 7044
RRIDAB_2135782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LEFTY2 antibody 13991-1-APDownload protocol
IHC protocol for LEFTY2 antibody 13991-1-APDownload protocol
IF protocol for LEFTY2 antibody 13991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Stroke

Mesenchymal Behavior of the Endothelium Promoted by SMAD6 Downregulation Is Associated With Brain Arteriovenous Malformation Microhemorrhage.

Authors - Weilun Fu
mouseWB

J Biol Chem

MicroRNA-127 Promotes Mesendoderm Differentiation of Mouse Embryonic Stem Cells by Targeting Left-Right Determination Factor 2.

Authors - Haixia Ma
ratIHC

Dev Biol

Role of EBAF/Nodal/p27 signaling pathway in development of placenta in normal and diabetic rats.

Authors - Qianqian Gao