Tested Applications
| Positive WB detected in | HepG2 cells, PC-3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12840-1-AP targets LGTN in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3579 Product name: Recombinant human LGTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 235-585 aa of BC001585 Sequence: DKSLSEAPEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRVKKADLPLLTSTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQIIQVKELSKGVESIVAVDWKHPRITSFVIPEPSPTSQTIQEGSREQPYHPPDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNKNLVRLDPILCDCILEKNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGRICPIDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHHLGWLLLEEYQLPRKHIQGLEKALKPGKKK Predict reactive species |
| Full Name | ligatin |
| Calculated Molecular Weight | 584 aa, 65 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC001585 |
| Gene Symbol | LGTN |
| Gene ID (NCBI) | 1939 |
| RRID | AB_2135302 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41214 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LGTN antibody 12840-1-AP | Download protocol |
| IHC protocol for LGTN antibody 12840-1-AP | Download protocol |
| IP protocol for LGTN antibody 12840-1-AP | Download protocol |
| WB protocol for LGTN antibody 12840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-eIF2D antibody (Cat# 12840-1-AP) has 22 citations across journals including Science, Nature Communications, Molecular Cell, Nucleic Acids Research, PNAS, EMBO Journal, Cell Reports, Genes & Development, PLoS Biology, Diabetologia, eLife, and others. Research fields span translation initiation, ribosome recycling, stress granule dynamics, ALS/FTD pathogenesis, viral IRES-dependent translation, endoplasmic reticulum stress, and perinatal cardiac function.
| Species | Application | Title |
|---|---|---|
Science Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.
| ||
Nat Commun A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.
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Mol Cell Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis. | ||
Nucleic Acids Res Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.
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Nucleic Acids Res eIF2D promotes 40S ribosomal subunit recycling during intrinsic ribosome destabilization.
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Nucleic Acids Res Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cells. |
