LGTN Polyclonal antibody

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  WB analysis using 12840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12840-1-AP (LGTN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12840-1-AP

  IP result of anti-LGTN (IP:12840-1-AP, 4ug; Detection:12840-1-AP 1:2000) with HepG2 cells lysate 1320 ug.

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  IHC staining of human stomach using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12840-1-AP

  Immunofluorescent analysis of HepG2 cells, using LGTN antibody 12840-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• Featured Product
• KD/KO Validated

LGTN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12840-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ligatin, Hepatocellular carcinoma-associated antigen 56, HCA56, EIF2D

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

12840-1-AP targets LGTN in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3579

  Product name: Recombinant human LGTN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 235-585 aa of BC001585

  Sequence: DKSLSEAPEDTSTRGLNQDSTDSKTLQEQMDELLQQCFLHALKCRVKKADLPLLTSTFLGSHMFSCCPEGRQLDIKKSSYKKLSKFLQQMQQEQIIQVKELSKGVESIVAVDWKHPRITSFVIPEPSPTSQTIQEGSREQPYHPPDIKPLYCVPASMTLLFQESGHKKGSFLEGSEVRTIVINYAKKNDLVDADNKNLVRLDPILCDCILEKNEQHTVMKLPWDSLLTRCLEKLQPAYQVTLPGQEPIVKKGRICPIDITLAQRASNKKVTVVRNLEAYGLDPYSVAAILQQRCQASTTVNPAPGAKDSLQVQIQGNQVHHLGWLLLEEYQLPRKHIQGLEKALKPGKKK

• Full Name: ligatin
• Calculated Molecular Weight: 584 aa, 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC001585
• Gene Symbol: LGTN
• Gene ID (NCBI): 1939
• RRID: AB_2135302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41214
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Science; Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.; Authors - Shelley R Starck ; KD Validated
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
rat	WB	Genes Dev; Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.; Authors - Maxim A Skabkin
human,mouse	WB	Nat Commun; A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.; Authors - Yoshifumi Sonobe ; KD Validated
human	WB	Nucleic Acids Res; Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.; Authors - Kazuya Ichihara ; KO Validated
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis